Sympatric versus allopatric evolutionary contexts shape differential immune response in Biomphalaria / Schistosoma interaction
Fig 1
Dual-RNAseq of Biomphalaria immune-related transcripts.
Among the differentially represented transcripts, Blast2GO functional annotation allowed us to identify 336 transcripts that appeared to be related to the Biomphalaria immune response. Abbreviations and colors: blue BB, sympatric interaction between BgBRE and SmBRE; green BV, allopatric interaction between BgBRE and SmVEN; and red BR, allopatric interaction between BgBRE and Srod. For each interaction 40 whole-snails are used, 20 pooled at 24h and 20 at 96h post-infection. A) Venn diagram showing the relationships among the immune transcripts found to be differentially expressed in the sympatric and allopatric interactions. B) Clustering of differentially represented immune transcripts. Heatmap representing the profiles of the 336 differentially represented immune-related transcripts in the BB, BV, or BR interactions along the kinetic of infection (at 24 and 96 h). Each transcript is represented once and each line represents one transcript. Colors: yellow, over-represented transcripts; purple, under-represented transcripts; and black, unchanged relative to levels in control naïve snails. C) Pie chart showing the distribution of the selected immune-related transcripts across three immunological processes: immune recognition (pink), immune effectors (brown), and immune signaling (blue). For each category and interaction, the respective proportion of transcripts and the direction of the effect (over- or underexpression) are indicated.