Transmission phenotype of Mycobacterium tuberculosis strains is mechanistically linked to induction of distinct pulmonary pathology
Fig 2
Increased inflammatory response and cellular infiltration in Mtb-LT infected mice.
Single cell preparations were obtained, and trypan blue exclusion method was used to calculate the total live cells in the lungs of Mtb-HT and Mtb-LT infected C3HeB/FeJ mice at 4 weeks post-infection (A). Lung cells from 4 week-infected mice were surface stained with directly conjugated antibodies to evaluate the total number of immune cell subsets by flow cytometry (B). Immunostaining with anti-Ly6G performed on paraffin-embedded fixed lung tissue sections from Mtb-HT and Mtb-LT infected mice. The image is a representative of three animals from each of the six infections. (C). For A and B, data are from five mice and presented as mean +/- SEM. One-way Anova was used to compare HT-1 infection with the rest of the groups.