A poxvirus pseudokinase represses viral DNA replication via a pathway antagonized by its paralog kinase
Fig 9
Subcellular localization of B12 and working model of B1/B12/BAF signaling during vaccinia infection.
(A) Vaccinia B1 kinase overlaps with cytoplasmic localized host BAF protein whereas vaccinia B12 pseudokinase shares a nuclear subcellular localization with the nuclear fraction of BAF. (B) The B1 kinase participates in restriction of BAF’s antiviral function against vaccinia DNA replication in the cytoplasm, while also repressing B12 negative regulation of vaccinia DNA replication through an unknown mechanism that is partly mediated via BAF regulation. Direct interactions and/or signaling through additional factors may be required for B1-B12 signaling and B12-BAF signaling, and are depicted using gold lines. B1-BAF interaction and BAF binding to dsDNA are direct interactions and denoted in black lines.