A poxvirus pseudokinase represses viral DNA replication via a pathway antagonized by its paralog kinase
Fig 8
The ΔB1mutB12 virus restriction of BAF antiviral activity is greater than the ΔB1 virus.
(A) Immunoblot analysis of total BAF protein (top panel) or phosphorylated BAF (bottom panel) in CV1 cells uninfected or infected with WT, ΔB1, ΔB1mutB12-A1, or ΔB1mutB12-A3 at a MOI of 10. Cells were collected at 6h post infection. (B) Control or CV1 cells expressing 3XFlag-tagged BAF in excess were infected with WT (black), ΔB1 (red), or ΔB1mutB12 (green) at a MOI of 3 and harvested at 24h post infection for analysis of relative DNA accumulation or (C) viral yield titration on CV1-B1myc cells.