A poxvirus pseudokinase represses viral DNA replication via a pathway antagonized by its paralog kinase
Fig 5
B12 reconstitution during infection with B1 and B12 naïve viruses repressed vaccinia replication.
(A) Immunoblot analysis of control or HA-B12 (GeneArt) lentivirus transduced CV1 cells was completed to detect tubulin (loading control) and HA (HA-tagged B12). (B) CV1 control cells or cells stably expressing B12 or HA-tagged B12 were infected with WT or ΔB1mutB12-A3 at 300PFU/well and fixed 72h post infection. (C) 24h relative DNA accumulation quantification was completed for CV1 control or HA-B12 expressing cells transfected with siCtrl or siB12 and infected with WT (black), ΔB1 (red), or ΔB1mutB12-A3 (green) at a MOI of 3.