A poxvirus pseudokinase represses viral DNA replication via a pathway antagonized by its paralog kinase
Fig 4
Rescue of DNA replication block using siB12 is specific for viruses lacking a functional B1.
(A) Diagram of hypothesis that B12 is either a general inhibitor of DNA replication or specific to B1 kinase mutant viruses. (B) CV1 cells treated with siCtrl of siB12 were infected with WT (black), ΔB1 (red), ts2 B1 mutant (pink), ts24 D5 mutant (blue), or ts42 E9 mutant (purple) at a MOI of 3 and harvested 24h post infection for quantification of relative DNA accumulation. Infections were carried out at 31.5°C, 37°C or 39.7°C to provide permissive, semi-nonpermissive and nonpermissive temperatures respectively for the temperature sensitive mutant viruses.