E6 proteins from high-risk HPV, low-risk HPV, and animal papillomaviruses activate the Wnt/β-catenin pathway through E6AP-dependent degradation of NHERF1
Fig 2
NHERF1 protein levels increase with increased cell density.
(A) Protein levels of endogenous NHERF1 increase with cell confluency. Keratinocytes retrovirally transduced with either vector or 16E6_WT were counted and plated at the indicated cell densities. As confluency increased, NHERF1 protein levels also increased, though still reduced in the presence of 16E6_WT. The means of triplicate independent experiments ± standard error are shown. (B) NHERF1 RNA levels are not changed by cell confluency or by the presence of 16E6_WT. Total RNA was extracted from keratinocytes retrovirally transduced with either vector or 16E6_WT and plated at the indicated cell densities. cDNA was reverse transcribed and NHERF1 RNA levels determined by qPCR. The means of triplicate independent experiments ± standard error are shown.