TDP-43 proteinopathy in Theiler’s murine encephalomyelitis virus infection
Fig 2
TMEV infection induces aggresome formation in rodent, but not human cells.
(A) Double immunofluorescent staining for TDP-43 and vimentin in DA-infected BHK-21 cells at 8 HPI. Cells have a large juxtanuclear structure covered by vimentin that represents an aggresome (arrowheads). TDP-43 is localized within the aggresome. (B) DA and GDVII infection in L929 cells leads to cytoplasmic mislocalization and aggregation of PTB1, while DAΔL and GDVIIΔL infections induce minimal mislocalization of PTB1 at this time. (C) HeLa cells infected by DA and GDVII show mislocalization of TDP-43, however, no aggresome is induced (arrowheads). DAΔL and GDVIIΔL infection do not induce TDP-43 mislocalization. Scale bars: 10 μm (A) and 5 μm (B, C).