Complete protection of the BALB/c and C57BL/6J mice against Ebola and Marburg virus lethal challenges by pan-filovirus T-cell epigraph vaccine
Fig 5
Complete protection of the C57BL/6J mice against Ebola and Marburg virus challenges by FILOcep1&2 vaccination.
A) A group of the C57BL/6J mice was vaccinated using the C1C2 and M1M2 3 weeks apart and the pattern of immunodominance for the 12 FILOcep1&2 peptide pools was determined in an IFN-y ELISPOT assay in the Oxford laboratory 1 week later (n = 4). B) In the Winnipeg laboratory, groups of the C57BL/6J mice were immunized with the candidate FILOcep1&2 vaccines or control vaccines expressing irrelevant eGFP and challenged by Ebola and Marburg viruses on day 35 (Table 1). Kruskal-Wallis test was used to determine the significance of variation among regimens for immunodominant peptide pool P4 and the P value is shown above. B) Four mice were killed and the induction of filovirus-specific T cells was confirmed in an IFN-γ ELSIPOT assay kits using the 4 immunodominant peptide pools P3, P4, P5 and P7. C) Eight mice in the FILOcep1&2 (blue) and 8 in the control eGFP (red) groups were challenged with 1000 LD50 of either mouse-adapted EBOV (Mayinga; left) or 1000 LD50 mouse-adapted MARV (Angola; right) virus and animals’ body mass was measured daily till day 14 post challenge (top) and survival was monitored until day 28 post challenge (bottom). The P values for survival used the Log-rank (Mantel-Cox) Test, n = 8.