INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana
Fig 7
Phospho-modified IDD4 versions exert a distinct transcriptional activity, SA accumulation and susceptibility against PstDC3000 hrcC- infection.
(A) Phospho-modified IDD4 lines show a distinct SAGT1 expression 4 hrs after flg22 treatment. (B) Pst DC3000 hrcC- infection levels in two independent IDD4-AA and IDD4-DD lines. Plants of three biological replicates (n = 30) were spray-inoculated with a bacterial suspension at OD600 0.2. Density of colony forming units (cfu) was analyzed 2 and 72 hours post inoculation (hpi). (C-D) GO term analysis of down-regulated (C) and up-regulated genes (D) in the IDD4-DD line (p≤0.05). (E-F) GO term analysis of down-regulated (E) and up-regulated genes (F) in the IDD4-AA line (p≤0.05). GO analysis was performed by using AgriGo (TAIR10). (G) Transcriptome compositions of IDD4-AA and IDD4-DD lines with respect to significant differentially regulated genes (p<0.05). Three main clusters generated illustrate a distinct and partly opposite expression of differentially expressed genes in the particular genotypes. (H-I) Quantitative analysis of free SA levels in WT, IDD4-AA and IDD4-DD lines (H) and as well as in WT and idd4 mutant plants (I) analysed by LC-MS/MS, before and 24hrs after Pst DC3000 hrcC- infection. Plants of three biological replicates were spray-inoculated with a bacterial suspension at OD600 0.2. Boxes represent the 25th and 75th percentiles and the inner rectangle highlight the median, whiskers show the SEM, and outliers are depicted by dots (Min/Max range). (A, B, H, I) Error bars show ± SEM, statistical significance was analyzed by Student’s test, letters above bars represent significance groups, p≤0.05.