INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana
Fig 2
Transcriptome composition of idd4, IDD4ox and idd4 flagellin22-treated lines associated with the characterization of pathogenicity-associated traits.
(A) Transcriptome comparison of WT and idd4 mutant with and without flg22 treatment. The original FPKM values were adjusted by normalized genes/rows and subsequently processed by hierarchical clustering by means of average linkage method using MeV4.0. Blue and yellow color indicates relatively low and high expression levels, respectively. For complete gene list see S1 Table. (B-E) Transcriptome composition and gene ontology annotations of up-regulated genes (p<0.05) in idd4 mock (B-C) and idd4 flagellin22 treated samples (D-E), GO terms were determined by using the AgriGo database (TAIR9, genomic locus). For complete gene list see S2 Table. (F) Evaluation of H2O2 levels by 3,3'- diaminobenzidine staining (DAB) in untreated idd4 mutants compared to WT. Scale bar = 2.75 mm. Boxes represent the 25th and 75th percentiles and the inner rectangle highlights the median, whiskers show the SEM, and outliers are depicted by dots (Min/Max range). Statistical significance was analyzed by Student’s test, asterisks indicate significant difference, ***p<0.001. (G) flg22-induced ROS burst assay of idd4, IDD4ox and WT plants, 5 week old plants were treated with 1 μM flg22 treatment for 45 min, values indicate mean ± SE, n = 36 (3 biological replicates). (H-I) Transcriptome composition and gene ontology annotations of down-regulated genes (p<0.05) in IDD4 gain-of-function mutant (pUBI10::GFP:IDD4). GO terms were determined by using the agriGo database (see also S2 Table).