Roles of GP33, a guinea pig cytomegalovirus-encoded G protein-coupled receptor homolog, in cellular signaling, viral growth and inflammation in vitro and in vivo
Fig 7
Pathological findings in r33- and Δ33-infected dams at 6 days p.i.
Formalin-fixed specimens prepared from the dams described in the legend for Fig 5 were histologically analyzed. (A, B) Severe inflammatory cell infiltration with hemorrhage was observed more frequently in the lungs of the r33-infected dams (A) than in those of Δ33-infected dams (B). (C, D) Severe inflammation was observed in the portal area in the liver of r33 virus-infected guinea pigs, and GPCMV-antigen-positive cells were found by immunohistochemical analysis (inset) (C). Minor inflammatory infiltration (arrow) was found in the liver of Δ33-infected guinea pigs (D). (E, H) HE staining shows intranuclear inclusion bodies in the lymph nodes of one of the r33-infected guinea pigs (E, arrows). Immunohistochemical analysis of cells demonstrated GPCMV-positive cells in the lymph nodes (H). (F, G, I, J) Intranuclear inclusion bodies (F, arrows) and many GPCMV-positive cells were found in the spleens of r33-infected guinea pigs (I) by HE staining and by immunohistochemical analysis, respectively. In contrast, no abnormalities or viral antigens were detected in the spleens of Δ33-infected guinea pigs (G, J).