Roles of GP33, a guinea pig cytomegalovirus-encoded G protein-coupled receptor homolog, in cellular signaling, viral growth and inflammation in vitro and in vivo
Fig 3
(A) GPL cells in 48-well plates were infected with GPCMV r33 and Δ33 at an MOI of 0.05, and culture supernatants were recovered and frozen at the indicated hours after infection. The viral titers (IUs) were determined by counting GFP-positive cell foci at 2 days after infection. Means and SEMs of the virus stocks prepared in triplicated wells are plotted for r33 (open circles) and Δ33 (closed circles). (B) Representative images of GFP-positive cells after infection of GPE-7 cells and macrophages (Mϕ) with r33 or Δ33 at an MOI of 10 (the virus titers were determined in GPL cells) at 24 hrs and 36 hrs p.i., respectively.