Defining cooperative roles for colonic microbiota and Muc2 mucin in mediating innate host defense against Entamoeba histolytica
Fig 5
Entamoeba histolytica (Eh) supresses Math1 mRNA expression in colonic goblet cells ex vivo and in vitro.
(A) Math1 expression heatmap of Math1GFP mice colons, the dotted line indicates the colonic loop ligation. (B) Each colon was divided into proximal and distal regions and GFP signal quantified. (C) Monolayers of LS 174T human goblet cells where either exposed to Eh for 30 min (m), 1 h, fixed Eh with glutaraldehyde 2.5% (Glut), or pre-treated with IL-1β. mRNA expression levels of transcription factor MATH1 were measured by qPCR and normalized with GAPDH levels. GFP: green fluorescent protein, AU: arbitrary units. n = 6, *P < 0.05, ***P< 0.001.