Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner
Fig 3
An acute host-pathogen checkpoint predicts the outcome of CFT073 bladder infection.
At 24 hpi, urine and serum was collected from mice infected with 108 CFU UTI89 or CFT073, and urine bacterial burden (A), pyuria (B) and serum cytokines (C-F) were assessed. Infection outcomes (chronic cystitis vs. resolution) were determined over the course of four weeks, and data are plotted according to the four week infection outcome. There were no statistically significant differences in the overall data (i.e. all UTI89-infected mice vs. all CFT073-infected mice) at 24 hpi. Data are combined from two to three independent experiments. Data points represent actual values for each individual mouse, zeros are plotted at the limit of detection, and bars indicate median values. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, Kruskal-Wallis test with Dunn’s multiple test correction. PMN, polymorphonuclear neutrophil.