Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate
Fig 6
In situ structure of gH/gL complex adjacent to prefusion gB.
(A) A series of slices in a tomogram showing a HCMV particle at different sections. (B) A 3D surface view from the HCMV particle in (A) with subtomographic average of prefusion and “postfusion” gB and gH/gL placed back on the viral envelope segmented from the tomogram. Black arrow indicates the gB/gH/gL complex. Unidentified glycoprotein densities are indicated as rods. Tegument proteins are shown as cyan densities. The recently published icosahedral reconstruction of capsid [11] was low-passed to 10Å, radially colored and placed back in its location. (C~F) The subtomographic average (C) showing a putative gH/gL complex adjacent to prefusion gB. The subtomographic average is also shown fitted with crystal structure of gH/gL (ribbon) (PDB: 5VOB) [17] in semitransparent surface viewed from side (D), top (E) and bottom (F).