Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate
Fig 4
Schematic illustration of the full-length HCMV gB.
(A) Mapping of domains to the full-length HCMV gB primary sequence (SS = signal sequence, MPR = membrane proximal region, TM = transmembrane domain, Endo = endodomain). Upper inset: in the prefusion (Pre) conformation, the sequence of the central helix in DIII resolved in the postfusion gB crystal structure (Post) is predicted to fold into two helices joined by a short loop around residues 498–500. Lower inset: predicted secondary structures of the sequence encompassing the MPR, TM and endodomain of gB in the prefusion conformation. (B) Helical wheel diagram of the first 15 amino acids of MPR (sequence in red dashed box in (A)), showing one side with a cluster of hydrophobic amino acids.