Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate
Fig 2
(A) A 10Å-thick slab from the tomogram of S2 Movie showing a digital slice of various HCMV particles, each comprising an outer envelope layer, an intermediate tegument compartment, and an icosahedral capsid inside. Though all wrapped a pleomorphic glycoprotein-decorated envelope, these particles differ inside: each virion (yellow arrows) containing a C capsid (with DNA genome) and non-infectious enveloped particle (NIEP) either an A capsid (empty, blue arrow), or a B capsid (containing scaffolding protein but no DNA, red arrows). (B) A zoom-in slice of a C capsid, with dsDNA duplexes resolved among the fingerprint-like pattern of the genome. (C) A slice from the particle indicated by the cyan arrow in (A) showing the unique portal complex (arrow) at one of the 12 vertices. (D) A zoom-in envelope region of the particle indicated by a yellow arrow in (A) showing the two resolved leaflets of the lipid bilayer envelope (inset: the enlarged boxed region). The side of the boxes in B-D is 120 nm.