Characterization of Haartman Institute snake virus-1 (HISV-1) and HISV-like viruses—The representatives of genus Hartmanivirus, family Arenaviridae
Fig 5
In vitro effects of hartmanivirus.
Primary cell line derived from juvenile B. constrictor kidney (I/1Ki). A-D) HISV-1 infection, 6 dpi. A) Overview; cells with extensive cytoplasmic vacuolization(arrows) and multiple electron dense nodules along the cell membrane (blebbing; arrowheads). B) Individual cell with cytoplasmic lamellar bodies (arrows), microtubuli (arrowheads) and cytoplasmic vacuolization. C) Individual cells with blebbing along the cell membrane (arrowheads) and large cytoplasmic vacuoles (asterisks). D) Higher magnification of cell membrane blebs. E) UGV-1 infection, 6 dpi. Cells with characteristic cytoplasmic electron dense inclusion bodies (asterisks) and mild cytoplasmic vacuolization. There is no evidence of blebbing along the cell membrane.