Multiple components of the nuclear pore complex interact with the amino-terminus of MX2 to facilitate HIV-1 restriction
Fig 6
Depletion of NUP214 and TNPO1 disrupts nuclear envelope accumulation of MX2.
(A) HeLa cells stably expressing MX2 bearing a C-terminal FLAG tag were transfected with siRNA targeting NUP214 and/or TNPO1 or a non-targeting siRNA (CTRL) as described in Fig 3, and then seeded onto glass coverslips. Localization of MX2, and endogenous NUP214 and TNPO1 was visualized by fluorescence confocal microscopy, using anti-FLAG, anti-NUP214, and anti-TNPO1 antibodies respectively. DAPI was used to stain the nuclei. Scale bar represents 20 μm. (B) Predominant cellular localization of MX2 was determined visually (blinded) using a 60x objective and an average of 100 cells, randomly selected (mean ± SD; *p-value < 0.05; paired t-test).