Zinc-dependent substrate-level phosphorylation powers Salmonella growth under nitrosative stress of the innate host response
Fig 4
Fructose bisphosphatate aldolase content.
(A) Fructose-1,6-bisphosphate aldolase (Fba) activity in Salmonella lysates. Where indicated, the lysates were treated with 100 μM of the zinc chelator TPEN. The Fba activity was normalized to WT samples. N = 12, mean ± S.E.M. (B) Effect of the supplementation of EG minimal media with 5 μM ZnCl2 on the Fba activity of log phase Salmonella. N = 8, mean ± S.D. (C) Fba activity in Salmonella treated for 5 min with 750 μM spermine NONOate. N = 8, mean ± S.D. *, **, *** p < 0.05, <0.01, <0.001, respectively, as determined by two-way ANOVA. (D) Growth of ΔznuB Salmonella after 16–20 h of culture in J774 cells. Where indicated, ΔznuB Salmonella were complemented with fbaA or fbaB genes heterologously expressed from the pBAD promoter. N = 8–16; mean ± S.D. ***, p < 0.001 as determined by one-way ANOVA.