A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity
Fig 5
scPOM-bi does not induce toxicity on CAD5 expressing PrPC in comparison to scPOM1.
The percentage of PI positive cells for different mPrP:antibodies complexes (A) or antibodies alone (B) on CAD5 PrPC (left) and on CAD5 Prnp-/- (right) are shown; each sample was added to cells after 10’ or 60’ of incubation at RT. The scPOM1:mPrP soluble oligomers caused toxicity in PrPC-expressing CAD5 cells but not in PrPC knock-out (PrP-/-) CAD5. No toxicity was detected when scPOM1 was in complex with the truncated ΔmPrP90-230 lacking the FT. Addition of the scPOM2:mPrP complex resulted in no toxicity, as well. Addition of the scPOM-bi:mPrP material to cells 10 minutes after complex formation resulted in toxicity, albeit lower than with scPOM1. However, toxicity was not significant if the material was added 60 minutes after complex formation. (n = 4 for all samples tested).