The trypanocidal benzoxaborole AN7973 inhibits trypanosome mRNA processing
Fig 2
Effects of AN7973 on the cell cycle and protein synthesis.
A) Cumulative growth curve for bloodstream-form trypanosomes incubated with and without AN7973. Results are arithmetic mean ± standard deviation for three replicates. B) Cell cycle analysis by microscopy. The percentages of cells with different numbers of nuclei and kinetoplasts are shown. C) Effect on protein synthesis. 5x106 Cells were incubated with AN7973 (10x EC50) for various times, then (after washing and 15-min pre-incubation) with [35S]-methionine for 30 min. Proteins were separated by SDS-PAGE and radioactive incorporation was assessed by autoradiography. The Coomassie-stained gel is shown as a loading control. Two prominent protein bands showing different effects of AN7973 are indicated as "a" and "b". All lanes are from the same gel and exposure; the gaps between the lanes are present because identical results for two partially resistant lines (also treated at 10x EC50) have been deleted.