Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway
Fig 2
Transcriptional analysis of NK cells in response to RBCs and iRBCs.
NK cells from five responders and five non-responders were co-cultured with either iRBCs or RBCs for 96 h. NK cells were purified from the four cultures for RNA isolation, cDNA library construction and microarray analysis. (A) sPLS-DA supervised clustering of the indicated conditions. (B) Pairwise comparison of all groups. (C) Heatmap of expression levels of identified DEGs. Each row represents a gene and each column represents a sample of the indicated group. Hierarchical clustering of columns and rows were performed using Euclidean distance and represented as a dendrogram. The organization and length of the branches in the dendrogram reflect similarities in gene expression profiles. (D) Top 10 pathways obtained from functional network-based analysis of the DEGs in R-iRBC samples.