Circulating HLA-DR+CD4+ effector memory T cells resistant to CCR5 and PD-L1 mediated suppression compromise regulatory T cell function in tuberculosis
Fig 9
PTB Teff containing HLA-DR+ subset are compromised in CCR5 and PD-L1 mediated signalling.
Sorted PTB total and HLA-DR- Teff cells were treated with 50 ng/ml each of CCL3 and CCL4 (A), 5 μg/ml anti-CCR5 (A), 5 μg/ml anti-PD-L1 (B) or 10 μg/ml recombinant PD-L1 (B). Cells were activated with anti-CD3/anti-CD28 beads (beads:cell ratio of 1:1). Anti-CCR5, anti-PD-L1 and recombinant PD-L1 were added 20 minutes prior to addition of mitogenic anti-CD3/anti-CD28. Unstimulated cells were used as control. NFκB activation was measured by ELISA after 180 minutes of stimulation and was expressed as absorbance at 450 nm. (A) shows data pertaining to CCR5 mediated signaling and (B) shows data pertaining to PD-L1 mediated signaling. Data shows median and maximum and minimum values from N = 6 PTB donors. Paired Wilcoxon matched-pairs signed rank test with Bonferroni correction was used to determine P value. *p ≤ 0.03.