Plant A20/AN1 protein serves as the important hub to mediate antiviral immunity
Fig 10
The Arabidopsis homologue of Pha13, AtSAP5, is induced by SA and involved in virus resistance.
(A) The expression of AtSAP5 were analyzed by qRT-PCR in the WT, AtSAP5 overexpression lines (AtSAP5-oe-4 and AtSAP5-oe-11), and RNAi lines (AtSAP5-RNAi-3 and AtSAP5-RNAi-7). Data represent mean ± SD; n = 3 biological replicates *, p<0.05, Student’s t-test compared to WT (B) The accumulation levels of Cucumber mosaic virus (CMV) were analyzed by qRT-PCR from leaves of WT inoculated with buffer (Mock), CMV-inoculated WT, or CMV-inoculated transgenic Arabidopsis. Data represent mean ± SD; n = 5 biological replicates *, p<0.05, Student’s t-test compared to WT (C) Disease symptoms of CMV inoculated WT or transgenic Arabidopsis. The experiments were repeated twice with similar results, and 5 plants were used in each inoculation. Photo of one representative plant from each inoculation taken at 9 days post inoculation are shown here; Scale bar, 1 cm. (D) Time-course expression of AtSAP5 in SA-treated wild-type (WT, Col-0) Arabidopsis. Expression level of AtSAP5 was analyzed by qRT-PCR from leaves at 1 and 6 h post-treatment. Water treatment was used as a mock control. Data represent mean ± SD; n = 3 biological replicates *, p<0.05, Student’s t-test compared to mock. Actin was used as an internal control for normalization.