Exploitation of nuclear functions by human rhinovirus, a cytoplasmic RNA virus
Fig 8
An SFPQ cleavage product associates with in vitro transcribed HRV16 RNA.
(A) HeLa cells were mock- or HRV16 infected, fractionated at 2 h intervals, and the subcellular distribution of proteins was analyzed by Western blot. The cleavage product (cp*) of polypyrimidine tract binding protein 1 (PTBP1) is indicated and HRV16 3D and its precursor 3CD served as markers of infection. VCL and LMNA served as markers for the cytoplasm (C), nucleus (N); GAPDH was used as a general loading control. (B) Biotinylated, in vitro transcribed control or HRV16 RNA were assayed for binding to cellular proteins present in HeLa cell lysates following mock infection or 8 hours post-infection (hpi) with HRV16 by Western blot analysis. A representative experiment is shown. (C) Quantification of four separate RNA affinity experiments was carried out using Quantity One software. Means are shown and error bars represent standard deviations (* P < 0.05).