Exploitation of nuclear functions by human rhinovirus, a cytoplasmic RNA virus
Fig 2
HRV16 induces a coordinated redistribution of proteins into the cytoplasm of infected cells.
Scatter plots show 8hpi:mock abundance ratios for all tryptic peptides detected in replicate in (A) nuclear and (B) cytoplasmic fractions. On the theoretical diagonal line of reproducibility (lower-left to upper-right), the apparent overrepresentation of tryptic peptides in the lower-left quadrant with respect to the upper-right for nuclear tryptic peptides is consistent with a net efflux of proteins from the nucleus, and the corresponding apparent overrepresentation of the upper-right quadrant for cytoplasmic tryptic peptides is consistent with a net influx of proteins into the cytoplasm. (C) Tryptic peptides scoring < 0.9, reproducibly, in nuclear 8hpi:mock abundance ratio were recorded (red) and the subset shared with both cytoplasmic replicates was then highlighted (bold red) on the cytoplasmic scatter plot (D), indicating a clear coordination between nuclear depletion and cytoplasmic enrichment.