HLA-C downregulation by HIV-1 adapts to host HLA genotype
Fig 5
Transmembrane domain residues of HLA mediate the interaction with Vpu.
(A) 293T cells were transfected with Flag-tagged constructs of Vpu from a primary virus 2_87, Vpu from NL4-3, or GFP as a negative control. Cells were co-transfected with HA-tagged constructs of HLA from the A or C loci, or HA-tagged β-TrCP as a positive control. Flag immunoprecipitation was followed by western blotting with anti-Flag or anti-HA antibody, and blots of the lysate prior to immunoprecipitation are also shown. (B) Chimeras 1–6 were constructed with the regions exchanged between HLA-A and HLA-C shown. (C) Co-immunoprecipitations were repeated using Vpu from 2_87 and the chimeric HLA constructs. (D) Sequences of the transmembrane domain of representative HLA-A and HLA-B alleles, 3 alleles which represent the transmembrane sequences of all common HLA-C alleles, and the chimeric constructs 5 and 6. Complete sequences for all HLA constructs are detailed in S8 Fig.