Mucosal immunoglobulins protect the olfactory organ of teleost fish against parasitic infection
Fig 9
Local IgT-, IgM- and IgD-specific responses in olfactory organ explants of survivor fish.
The olfactory organ, head kidney and spleen explants (~ 20 mg each) from control and survivor fish were cultured in medium (400 μl) for 7 days. Immunoblot analysis of IgT-, IgM- and IgD-specific binding to Ich in the culture medium of olfactory organ (A), head kidney (B) and spleen (C) (dilution 1:2) from control and survivor fish. (D-F) IgT-, IgM- and IgD-specific binding to Ich in dilutions of culture medium from olfactory organ (D), head kidney (E) and spleen (F) from control and survivor fish, measured by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 6–8 per group). *P < 0.05, **P < 0.01 and ***P < 0.001 (unpaired Student’s t-test). Data are representative of at least three independent experiments (mean and s.e.m.).