Mucosal immunoglobulins protect the olfactory organ of teleost fish against parasitic infection
Fig 7
Immunoglobulin responses in the nasal mucus and serum from infected and survived trout.
(A) Western blot analysis of IgT-, IgM- and IgD- specific binding to Ich in nasal mucus (dilution 1:2) from infected and survivor fish. (B and C) IgT-, IgM- and IgD- specific binding to Ich in dilutions of nasal mucus from infected (B) and survivor (C) fish, evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 8 per group). (D) Western blot analysis of IgT-, IgM- and IgD- specific binding to Ich in serum (dilution 1:10) from infected and survivor fish. (E and F) IgT-, IgM- and IgD- specific binding to Ich in dilutions of serum from infected (E) and survivor (F) fish, evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 8 per group). *P < 0.05, **P < 0.01 and ***P < 0.001 (unpaired Student’s t-test). Data are representative of at least three independent experiments (mean and s.e.m.).