Mucosal immunoglobulins protect the olfactory organ of teleost fish against parasitic infection
Fig 2
Trout pIgR associates nasal sIgT.
(A) SDS-PAGE under reducing conditions of trout serum and nasal mucus (~ 5 μg each), followed by immunoblot analysis using anti-trout pIgR antibody. (B) Co-immunoprecipitation (CoIP) of nasal mucus with anti-trout IgT antibody, followed by immunoblot analysis under reducing conditions (pIgR detection, upper panels) or non-reducing conditions (IgT detection, lower panels). (C) Immunofluorescence staining for pIgR with IgT in olfactory organ paraffinic sections of rainbow trout. Differential interference contrast images of olfactory organ paraffin sections were stained with anti-trout pIgR (magenta), anti-trout IgT (green) and DAPI for nuclei (blue) (n = 9) (isotype-matched control antibodies for anti-pIgR in S1A Fig). Enlarged sections of the areas outlined showing some pIgR/IgT colocalization (white arrowhead). NC, nasal cavity; OE, olfactory epithelium; LP, lamina propria. Scale bar, 20 μm. Data are representative of at least three different independent experiments.