Enzymatic chokepoints and synergistic drug targets in the sterol biosynthesis pathway of Naegleria fowleri
Fig 5
Synergistic effect of drugs at low concentrations.
The phase contrast microscope images show N. fowleri trophozoites treated for 48 hours with (A) 0.08 μM of isavuconazole and 0.7 μM of epiminolanosterol, (B) 1.9 μM equimolar concentrations of isavuconazole and tamoxifen, (C) 2 μM equimolar concentrations of epiminolanosterol and tamoxifen, and (D) 0.5% DMSO, which served as a negative control. The inhibitor-treated N. fowleri cells visible in the microscope field are rounded, much smaller in size and not viable, whereas DMSO-treated cells are irregularly shaped with visible cytoplasm. Magnification, ×20.