Enzymatic chokepoints and synergistic drug targets in the sterol biosynthesis pathway of Naegleria fowleri
Fig 2
Gas chromatography of the total sterol fractions derived from N. fowleri trophozoites treated with (A) 0.5% DMSO, (B) 0.2 μM posaconazole, (C) 5.4 μM epiminolanosterol, and (D) 5.6 μM AY9944. Peaks are labeled with numbers corresponding to metabolites listed in Table 1. Insert in (C) shows deconvolution of the major peak resulting from an overlap of three different sterols: 6, m/z = 349; 7, m/z = 363 and 13, m/z = 400. The color scheme is the same as in Fig 1: CYP51 inhibitor (shown in green), SMT inhibitor (in blue) and ERG2 inhibitor (in dark red).