Diverse pathways of escape from all well-characterized VRC01-class broadly neutralizing HIV-1 antibodies
Fig 2
Soft randomization introduces a controlled number of amino-acid substitutions into a specific target region.
(A) The distributions of the number of amino-acid substitutions in the Loop D and V5 regions are shown. Pie charts are generated based on the data from approximately 600,000 next generation sequence reads. (B) Amino acid substitutions in Loop D and V5 regions of the parental virus library are shown in 2-dimensional heat maps. The relative frequency of the substitutions of each residue is represented by a red gradient. The original amino acids of wild-type ADA Env are indicated as a square with a diagonal line.