Macrophages inhibit Aspergillus fumigatus germination and neutrophil-mediated fungal killing
Fig 6
A CEA10-derived strain is cleared more efficiently by macrophage-deficient hosts than wild-type hosts.
A, B. Macrophage-deficient (irf8-/-) or control (irf8+/+) larvae were infected with TBK1.1 (Af293) or TFYL49.1 (CEA10) and CFUs (A) or larval survival (B) were monitored. For CFU experiments, average injection CFUs: Af293 = 23, CEA10 = 23. For survival experiments, average injection CFUs: Af293 = 44, CEA10 = 31. C, D. Macrophage-depleted (clodronate liposomes) or control (PBS liposomes) larvae were infected with TBK1.1 (Af293) or TFYL49.1 (CEA10) and CFUs (C) or larval survival (D) were monitored. Average injection CFUs: Af293 = 37, CEA10 = 40. For all analyses, data shown are from 3 pooled replicates. For CFU analyses, data represent lsmeans ± SEM, P values calculated by ANOVA. All CFU data are from 24 larvae (3 replicates, 8 larvae each) per condition per day. For survival analyses, P values were calculated by Cox proportional hazard regression analysis. See also S6 Fig.