Invasion of midgut epithelial cells by a persistently transmitted virus is mediated by sugar transporter 6 in its insect vector
Fig 3
LsST6 is involved in rice stripe virus invasion of the midgut epithelial cells of L. striatellus.
(A) LsST6 expression in different tissues. Total RNA was extracted from the salivary gland, gut, hemolymph and ovary for assay by RT-qPCR. The whole insect body served as the control. Means ± SEM of three independent experiments are shown (P < 0.01; one-way ANOVA, least significant difference [LSD] test). WB: whole body; SG: salivary glands; Gu: gut; He: hemolymph; Ov: ovary. (B) Localization of LsST6 in midgut epithelial cells. Excised midgut was incubated with anti-LsST6 antibody labeled with Dylight 488 (green) and observed with LSCM. Dylight 633 phalloidin was used to label actin (blue) of midgut epithelial cells. Scale bars, 50 μm. (C-E) Colocalization of LsST6 and RSV on membrane of midgut epithelial cell during RSV invasion. Excised midguts were incubated with anti-LsST6 labeled with Dylight 488 (green) and anti-RSV labeled with Dylight 549 (red) at 2 days (C), 4 days (D) and 8 days (E) after a 2-day access acquisition period (AAP), then observed with CLSM. Scale bars, 50 μm.