Immobility in the sedentary plant-parasitic nematode H. glycines is associated with remodeling of neuromuscular tissue
Fig 6
hg-UNC-25 synthesizes GABA in H. glycines.
The hg-unc-25 cDNA from H. glycines partially rescues the C. elegans unc-25(e156) mutant. In wild-type C. elegans, GABAergic neurons are detected in the head and ventral nerve cord (arrowheads) using anti-GABA staining. In the C. elegans unc-25(e156) mutant, GABA is not produced and GABAergic neurons cannot be detected using anti-GABA staining. A plasmid construct containing hg-unc-25 cDNA driven by the C. elegans unc-25 promotor partially rescues the C. elegans unc-25(e156) mutant as seen by GABA-immunoreactivity (arrowheads). Arrows indicate the expression of coel::RPF used as a co-injection marker. Scale bars, 10 μm.