Metabotropic glutamate receptor subtype 2 is a cellular receptor for rabies virus
Fig 1
siRNA silencing and overexpression of mGluR2 affect RABV infection of cells.
(A) Schematic for mGluR2 protein. Transfection with siRNA s6195 resulted in the downregulation of mGluR2 mRNA in transfected HEK293 cells (B), SK cells (C), and N2a cells (D). Silencing of mGluR2 inhibited ERA-eGFP infection of HEK293 cells (E), SK cells (F), and N2a cells (G). NT, non-targeting siRNA. (H) Overexpression of mGluR2 enhanced the growth of ERA-eGFP at a multiplicity of infection (MOI) of five in transfected HEK293 cells; virus titers in the cell culture supernatant were determined as focus forming units (FFU) in BSR-T7/5 cells. A one-way ANOVA was used for the statistical analysis. *, p<0.05. **, p<0.01.