KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis
Fig 8
Tyrosine-kinase truncated dominant-negative mutants of PDGFRA block KSHV tumorigenesis in mice.
(A) Structure of the truncated dominant-negative forms of PDGFRA and PDGFRB. (B) Phosphorylated PDGFR and total PDGFR determined by immunoblotting in mECK36 cells transfected with dominant-negative forms of PDGFRA, PDGFRB, or empty vector control stimulated with either PDGF-AA (80 ng/mL) or PDGF-BB (20ng/ml) for 10 min. (C) Tumor growth curve from mice following subcutaneous injection of mECK36 cells transfected with dominant-negative forms of PDGFRA, PDGFRB, or empty vector control. Data indicate mean tumor size ± SD (n = 10).