PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves
Fig 9
CD206 colocalizes with Schwann cells in nerve lesions of leprosy patients.
Nerve biopsies were labeled with antibodies for the SC-specific marker S100 (red image), and the mannose receptor CD206 (green image) and then visualized by fluorescence microscopy. Nuclei were labeled with DAPI (blue image). A-D. Serial sections of a leprosy patient nerve biopsies were analyzed. A. Wade staining showing M.leprae. Scale bars, 20μm. B-D. Tissue sections were visualized by fluorescence microscopy showing CD206/S100 co-staining SCs. Images are representative of a total of five patients. E-G. Serial sections of a nerve biopsy from a patient with a non-leprosy peripheral neuropathy. The merged image in G. shows no CD206/S100 co-localization.Images are representative of a total of three patients. Scale bar, 20μm. Insets: magnified views of CD206/S100 staining in leprosy (insets 1 and 2) and non-leprosy (insets 3 and 4) nerve biopsies. Scale bar, 10μm.