PGL I expression in live bacteria allows activation of a CD206/PPARγ cross-talk that may contribute to successful Mycobacterium leprae colonization of peripheral nerves
Fig 8
Crosstalk between PPARγ and CD206 mediates IL-8 production in Schwann cells infected with PGL I-producing mycobacteria.
A. SCs were transfected for 24 h with control siRNA or siRNA targeting mrc1, followed by infection with M. leprae or BCG PGL I for 48 h. Supernatants were analyzed for IL-8 production by ELISA. B and C. Alternatively, cells were pretreated with mannose 100 μg/mL or the PPARγ antagonist GW9662 (5 μM) respectively, 30 minutes before infection. Cells were then infected with M. leprae or BCG PGL I for 48 h. Supernatants were analyzed for IL-8. The results are the mean ± SEM from at least three independent experiments performed in triplicate. Statistical significance was calculated by ANOVA followed by Bonferroni’s multiple comparison test. *p < 0.05; ** p<0.01; ***p<0.001.