Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition
Fig 7
mar1Δ cells have disordered cell walls and altered vesicular trafficking by ultrastructure analysis.
WT and mar1Δ cells were incubated for 16–18 hours in YPD (30°C) and TC (37°C) medium, followed by glutaraldehyde and KMnO4 fixation and partial dehydration as described previously [14,68,101]. Samples were further processed, embedded, sliced, and imaged by transmission electron microscopy (TEM). (A) YPD incubated cells display thin, ordered cell walls. Left image of each pair: bar, 1 μM. Right image of each pair: bar, 200 nm. Letters indicate location of inset. (B) mar1Δ cells incubated in TC medium have a less organized cell surface. Left image of each pair: bar, 1 μM. Right image of each pair: bar, 200 nm. Letters indicate location of inset. Red arrows indicate apparent cell wall material disassociating from cell surface. (C) There are increased numbers of electron lucent structures in mar1Δ cells incubated in TC medium. Bar, 1 μM. Red asterisks indicate mar1Δ cells with several electron lucent vesicles near cell periphery.