A cell-based infection assay identifies efflux pump modulators that reduce bacterial intracellular load
Fig 11
EPM35 and EPM43 block efflux of Nile red from ESKAPE MDR clinical isolates.
(A-D) Defined strains obtained from BEI resources were examined for Nile red retention after glucose addition in the presence of the indicated compound. Data for each sample were normalized to the initial fluorescence (100%). Dose response curves at seven minutes after glucose addition. Mean of three biological replicates performed in duplicate. * p < 0.05; ** p < 0.01; *** p < 0.001; ****p < 0.0001 compared to DMSO + glucose by one-way ANOVA and Dunnett’s multiple comparison post-test.