A cell-based infection assay identifies efflux pump modulators that reduce bacterial intracellular load
Fig 7
EPM35 and EPM43 interact with the efflux pump AcrB.
(A, B) Representative ITC for the binding of (A) EPM35 and (B) EPM43 to E. coli AcrB. Each peak in the upper graphs corresponds to the injection of 2 μL of 100 μM of the EPM in buffer containing 20 mM Na-HEPES (pH 7.5), 0.05% DDM and 5% DMSO into the reaction containing 10 μM of E. coli monomeric AcrB in buffer containing 20 mM Na-HEPES (pH 7.5), 0.05% DDM and 5% DMSO. The lower graphs show the cumulative heat of reaction displayed as a function of injection number. The solid line is the least-square fit to the experimental data.