Caspase-11-dependent pyroptosis of lung epithelial cells protects from melioidosis while caspase-1 mediates macrophage pyroptosis and production of IL-18
Fig 7
IFNγ stimulates ROS production that protects from B. thailandensis infection.
Mice were infected intranasaly with B. thailandensis (105 CFU). (A) ROS generation was measured ex vivo in neutrophils or macrophages obtained from BALF of mice of shown genotype 14 or 48 hours p.i., respectively. (B) Mice were sacrificed 48 hours p.i. and the organ bacterial burdens were measured. (C, D) Wild type mice were infected intranasaly with B. thailandensis (105 CFU) and treated daily with IFNγ or NAC. Mice were sacrificed 48 hours p.i. and the organ bacterial burdens (C) or cytokine levels in BALF (D) were measured. One representative experiment of two is shown. Data are expressed as mean ± S.D. *p<0.05, **p<0.01, ***p<0.001 (A, D) Unpaired t-test, (B, C) Mann-Whitney U test.