Caspase-11-dependent pyroptosis of lung epithelial cells protects from melioidosis while caspase-1 mediates macrophage pyroptosis and production of IL-18
Fig 6
IFNγ protection in melioidosis depends on ROS generation.
(A) Mice were infected intranasaly with B. thailandensis (105 CFU) and treated with IFNγ. Mice were sacrificed 48 hours p.i. and the organ bacterial burdens were measured. (B) BMM were infected with B. thailandensis (MOI 50) in presence or absence of IFNγ (100 ng/ml)). Cells were lysed 4 hours later and intracellular bacteria growth was measured (left panel). Induction of pyroptosis was measured as LDH release in conditioned supernatants (right panel). One experiment representative of three is shown. Data are expressed as mean ± S.D. *p<0.05, **p<0.01, ***p<0.001 (A) Mann-Whitney U test (B) One-way ANOVA.