PPARγ is critical for Mycobacterium tuberculosis induction of Mcl-1 and limitation of human macrophage apoptosis
Fig 4
M.tb induces Mcl-1 in a PPARγ- and 15-LOX-dependent manner.
A and B) MDMs were transfected with PPARγ (siP) or scrambled control (sc) siRNA then infected with M.tb at MOI 5 for 24 h. C-F) MDMs were treated with the indicated LOX inhibitors (μM) 1 h before, and during, M.tb infection (MOI 5 for 6 h). G and H) MDMs were treated with PD146176 (25 μM) 1 h before, and during, M.tb infection (MOI 5 for 6 h); along with 13-HODE (30 μM). A-H) MDMs were lysed and protein analyzed by Western blot, and densitometry analysis was conducted with Image J. Data are expressed as amount of Mcl-1 relative to M.tb infected scrambled control (B) or infected no inhibitor control (D, F, H) and are the cumulative mean ± SEM of at least 3 experiments, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.