PPARγ is critical for Mycobacterium tuberculosis induction of Mcl-1 and limitation of human macrophage apoptosis
Fig 1
Identification of novel PPARγ effectors with NanoString.
MDMs were transfected with PPARγ (siP) or scrambled control (sc) siRNA, then infected with M.tb at MOI 5 for 6 and 24 h. A) Representative Western blot showing knockdown efficiency, mean knockdown efficiency was 81.7 ± 5.5% (N = 3). B and C) Total RNA was extracted and NanoString analysis was performed with a Human Immunology Panel. Shown are genes that displayed a significant (p < 0.05) mean fold change of at least 1.5 after PPARγ knockdown and 6 (B) or 24 (C) h of infection. Asterisks in C indicate Bax and Mcl-1. N = 3. D and E) Total RNA was collected and gene expression analyzed by qRT-PCR. Results are expressed as Bax (D) and Mcl-1 (E) expression relative to the scrambled control cells and are the mean ± SEM of 3, in triplicate, * p < 0.05.