Vaccine-induced antibodies to herpes simplex virus glycoprotein D epitopes involved in virus entry and cell-to-cell spread correlate with protection against genital disease in guinea pigs
Fig 5
Antibody passive transfer of prototype MAbs in mice.
BALB/c mice were passively immunized IP and infected the next day intravaginally with 5x103 PFU HSV-2 strain MS. Animals were observed for 10 days for survival (A), weight loss (B) and genital disease (C). Weights recorded are for surviving animals, which explains the increase in average weights observed in many groups towards the end of the experiment as euthanized animals drop out. Sample size: n = 15 for non-immune IgG; n = 10 for each prototype MAb; and n = 10 for uninfected animals. The Mantel-Cox method was used to calculate P values for survival curves. Each prototype MAb provided significantly better protection than non-immune IgG (P values noted on Fig 5A), and each prototype MAb provided significantly better protection than MC16 (p<0.001). Protection provided by DL11 or MC2 (both protected 80% of mice) was significantly better than MC23 (protected 30% of mice) (p = 0.036). No significant differences were detected for weight loss in any group. Significant differences between prototype MAbs and non-immune IgG for genital disease scores are noted in Fig 6C. Student’s t-tests were used to compare MAbs with nonimmune IgG for weight loss and disease scores. * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001.